DNA DAMAGE AS APOPTOTIC FACTOR IN HUMAN MESENCHYMAL STEM CELLS DURING TOXOPLASMA GONDII INFECTION

Abstract

Toxoplasma gondii is a parasitic worm that infects all warm-blooded creatures on the planet. When it infects a cell, it manipulates the host's DNA damage response, which is still unknown. The study's goals were to I analyze DNA damage in T. gondii-infected cells in vitro, and (ii) figure out what causes DNA damage in T. gondii-infected cells effects of T. gondii-induced apoptosis and DNA damage in human umbilical cord mesenchymal stem cells were investigated using DNA fragmentation analysis. The extent of DNA damage was measured using the comet test to determine the level of DNA damage. This study included three groups: control, nutritionally infected, and in vitro infected. Infected hMSCs showed a considerable increase in DNA damage in the comet tail when compared to the control group. In vitro infected samples had substantially more severely damaged spots than naturally infected samples. T. gondii infection caused DNA damage in infected MSCs.