Antibiotic Resistance of Strong Biofilm Forming Bacteria Isolated from Different Oral Samples of Patients with Dental Problems

Abstract

Globally, the wide use of antibiotics in human treatments, animal food supplements, and agriculture practice was the main cause of the emergence of multi antibiotics resistant strains of bacteria which led to a critical health problem. The purpose of this study was to reviewing the antibiotics resistance ability of strong biofilm former isolates isolated from saliva, teeth and necrotic roots canals. Biofilm formation assay was done by using Microtiter plate method (MTP), among 267 bacterial isolates (137 from saliva, 88 from teeth and 42 from necrotic roots canal), 104 isolates (55 from saliva, 32 from teeth and 17 from necrotic roots canals) were considered as strong biofilm former and were screened according to its antibiotics resistance ability. The results showed that almost all the isolates can form visible growing biofilms when compared to the controls, though with difference in the degree of adhesions (strong, moderate and weak). Enterococcus sp. isolated from saliva, teeth and necrotic roots canals was the strongest biofilm former bacteria and as well as the most antibiotic resistant isolates. Further, the study revealed that antimicrobial resistance of bacteria isolated from saliva, teeth and necrotic roots canals of patients with dental caries conditions is associated with biofilm formation.