Bioactivity of natural compounds Produced by Marine Alcaligenes faecalis as Antimicrobial, antibiofilm formation and antibiocorrosion effect against Desulfovibrio sp. Isolated from crude oil fluid

Abstract

Microorganisms extract have served are source of valuable diverse molecules in drug industries. This study successfully identified potentially new sources of antibacterial compounds against sulfate reducing bacterium, Desulfovibrio sp. , SRB) ) which was isolated from crude oil field , PETRONAS Refinery , Melaka) Sdn. Bhd.) from Malaysia, and thus provide in protecting carbon steel coupons surface against biocorrosion caused by SRB present in crude oil .The marine , Alcaligenes faecalis) grown in marine broth extract , AF) were dissolved in chloroform , C).Chloroform extraction of A. faecalis , AFC) grown in marine broth was carried out to produce crude of A. faecalis. AFC was diluted at concentrations 0.2–12.8 mg/mL and was tested for antimicrobial activity by microdilution susceptibility tests in 96-wells plate. Using crystal violet assay method for confirm anti-biofilm activity. Inhibit the biocorrosion analyses were carried out by using weight loss. AFC was then analyzed by Gas Chromatography Mass Spectrometry, GC-MS). The results exhibited that, AFC achieved high significant effect for inhibiting growth in MIC 3.2 mg / ml against the concerned SRB. There were no biofilm formation or corrosion observed on carbon steel coupons after treatment with AFC were effective in controlling planktonic and sessile SRB growth. The GC-MS analysis showed the presence of 20 different chemical compounds in AFC, The major components in AFC can be related to antimicrobial, antifungal, antioxidant, pesticide, metabolism, toxicity, anticancer and corrosion inhibition activities. In conclusion, crude chloroform extract of A. faecalis has the ability to inhibit SRB growth and hindrance of biofilm formation and H2S production which led to biocorrosion