ANTIOXIDANT ACTIVITIES FROM LEAVES OF ELEPHANTOPUS SCABER L. EXTRACTED USING DIFFERENT SOLVENTS

Abstract

ABSTRACT : The present study was carried out to evaluate the antioxidant activities of leaves of Elephantopus scaber by using different solvent (ethyl alcohol, acetone and aqueous). The leaves extract from Elephantopus scaber using different solvents to extract were evaluated for antioxidant activity by using DPPH method using a different concentrations (1, 10, 20, 30, 40, 60, 80, 100 µg/ml). The results of this method was compared with ascorbic acid as standard. The IC50 value of standard (ascorbic acid) was found to be 20 mg/ml. This study showed the strongest DPPH radical-scavenging activity of (52.54±0.81%) inhibition at concentration of 40 mg/ml and IC50 value was 30 mg/ml was found the scavenging activity of (44.92±0.26%) for ethyl alcohol extract. Acetone extract of leaves was found at of concentration 60 mg/ml scavenging activity of 53.26±0.34%. The IC50 value of aqueous extract of leaves was (60µg/ml) with percentage inhibition of (54.28±0.28%) followed by (44.57±0.53%) at concentration 40 mg/ml.