The Use of The Genetically Modified of Azotobacter vinelandii In Isolate with the preparation of The Bio-organic Fertilizer From Local Materials and then Bio-testing It

Abstract

It is difficult to find an isolate that can give all plant needs , therefore it requires more than one isolate to give the various needs of the plant . The use of a mixture of isolates that can fix nitrogen and dissolve phosphate in a certain vaccine results decrease in their activity ,therefore the idea of using the isolate , Azotobacter vinelandii which is modified to dissolve phosphate and fix nitrogen in the preparation of local materials to make a compose is suitably fit to enhance the conditions of plant growth and soil features. The isolate ability on fixing nitrogen and dissolving super phosphate ( T-CaP ) or phosphate rocks ( RP ) compounds was tested by their abilities to give sidrophores and indol compounds .A mixture of various qualitative and quantitative components of local plant and animal , other natural minerals consisted of cow organic matter ( C/N = 27/1 ) dried materials of the plant , Ceritophylium demeresm ,two other phosphate rocks , natural apatite ( Rp-N )( %8P ) and calcinated phosphate apatite ( RP-C ) ( %12P ) was prepared .The aim was to change these components into a more useful mixture .A bacterial vaccine, A. vinelandii that was modified by fermintating the mentioned components on different time intervals 10 , 20 and 30 days before one day of their addition to the soil in a fermented and unfermented nature to change soil characters .Four fermented and unfermented mixtures were used mixed 1% of the used soil .Two fermented and non-fermented mixtures with 1% were selected as compared to the use of TSP and Urea fertilizer in pot experiment planted with Vicia catiang . Results indicated that the used isolate was able to fix nitrogen with an average of 2.8 mg/L with a microbial density of 6.2 × 108 Cfu/g indol ,dissolved phosphate compounds ( TCaP) with an average of 10.6 mg/L and produced indol components with and an average of 11.6 mg/L . The mixtures of 300 and 700 mg of RP-N and OM was found to the best in containing the dissolved phosphate compound , 71.2% from the total phosphorus and the total nitrogen 19.6 gm N/kg mixture which was the best average for the C/N in the mixtures after fermentation 1:9 . The use of the natural phosphate rocks gave the best dissolving results and nitrogen quantity was increased with the use of C. demeresm plant wastes .The use of the fermented mixtures enhanced soil texture after 30 days of incubation with an average of 30 mg of dissolved phosphorus /kg soil and 150 mg nitrogen,also rapid germination were achieved with an 277 average of 71 and 20.2 for plant and root length respectively The average root and vegetative dry weights was 160 and 178 g/plant respectively .Those treatments showed a better rhizopeal bacterial infection for plant roots with an average of 22 effective knots with 68% with protein percent 17.9 % in dried weight of the plant . Also ,they enhanced the soil content with viable phosphorus and nitrogen with an average of 20.5 and 130 mg/kg soil respectively .These results assure the existence of various carbon resources for microbial growth and activity resulted from the fermented mixtures which , in the mean time increased the fixation of nitrogen and dissolving phosphate compounds and finally producing Sidrophores , growth regulators , enhancing soil texture and viability of nutrients to the plants .