ISOLATION AND IDENTIFICATION OF AMYLASE PRODUCING BACTERIA FROM SOILS USING LOCAL MEDIA

Abstract

ABSTRACT: The study aimed at isolation starch hydrolyses bacteria and amylaseproducing bacteria from Ramadi soils to get efficient local isolates that produce the enzyme .30 bacteria isolates that produce the enzyme could be obtained; the (10) most efficient of which were selected. Cultural media containing plant wastes prevailing in the local environment were used. There are considered pollutants that are very cheap in the production of the enzyme. They are corn cap, clover hay Trifolium alexandrinum L., and shamblan Ceratophyllum demersum L. . The results have shown that the best production of the enzyme using starch agar was at pH7 and 35c temperature after 24 hours of incubation with the use of corn cap then shamblan as a source of carbon where the diameter of the clear zone area was 8cm from the local isolate (PR10). The local isolate (PR10) showed high ability to produce amylase enzyme using liquid cultures. The best production was at pH7 and 40c temperature using corn cap of2% concentration and incubation with the shaking incubator at 150 rpm and 1.5% inoculums concentration. The activity of the enzyme was 24.731 unit/ml using peptone as a source of nitrogen after incubation for 24 hours. Identification results of (PR10) isolate showed that it is B.subtilis bacteria