ISOLATION AND IDENTIFICATION BACILLUS CEREUS FROM RAMADI HOSPITALS AND STUDYING SOME HISTOPATHOLOGICAL CHANGES IN INFECTED RABBITS

Abstract

Three hundred fifty one samples were collected from Al-Ramadi hospitals. The samples were cultured in Lab. to investigate the presence of Bacillus spp. bacteria. This study was included the collection of samples from different routes in the Al-Ramadi city hospitals, Including patients wards, operation wards, labour wards, emergency wards, pederastic wards, burn and fractures wards, and reception wards. In the hospitals the samples was collected from grounds, walls, beds, patients bodies, medical staff, and visitors by using swabs. One hundred forty bacterial Isolates was Bacillus cereus; 57.8% (81 Isolates) was obtained, Bacillus subtilise; 25.67% (36 Isolates) was obtained, and Bacillus megaterium; 16.42% (23 Isolates). It was the higher percents of isolation from Al-Ramadi hospitals from maternity and childbed in percent 43.1%, followed by General Ramadi hospital in percent 38.5%, while Al-Rasheed local hospital in percent 32.6%. All Isolates showed the ȕ-Lactamase resistance (100%) which were included pencillins and cephalosporins. They were sensitive for the following antibiotics; Gentamycin, Vancomycin and Chloramphinecol (100%, 90%, and 80%, respectively) on frequently, Erythromycin were recorded less effect in these bacteria in percent 60%. All Isolates were showed the Capability on the production of ȕ-Lactamase enzymes, Bacillus cereus was recorded the higher percent in production of this enzyme in percent 92.60%, followed by Bacillus subtilise; 83.33%, then Bacillus megaterium; 82.60%. A minimum inhibitory concentration (MICs) of Bacteria Isolates was detrimented, It was showed that high (MICs) of Isolates was to the Gentamycin antibiotic in average 71.72%, followed by Chloramphenicol 66.65% ,then Vancomycin antibiotic 65.08%, and Amikacin antibiotic was recorded the less average 54.16%. Some Bacillus cereus Isolates were showed histopathologic changes on selected Newzaeland rabbits as model animal for studying after their inoculation of 72 hours by appearance of redness and ulcerations in the area of injection. Histopathologic sections were showed necrosis of hepatic and bowel tissues, also present of bleeding in the bowel epithelial tissues with destruction of epithelial tissue cells. Fatty changes were happened in the hepatic tissue and migration of inflammatory cells of hepatic and bowel cells, as significant of bacterial Isolates as they were pathogenic.