Single cell protein production by soil Raoutella ornithinolytica incubated on waste potato, paper and corn cob products.

Abstract

Al-Hadithi OAH, Al-Rawi DF, Abed Al-Ani MQ., Single cell protein production by soil Raoutella ornithinolytica incubated on waste potato, paper and corn cob media, Onl J Vet Res., 22 (12):1137-1144, 2018. We describe single cell protein (SCP) production by Raoutella ornithinolytica bacteria isolated from soil and cultured on potato, paper and corncob starch waste residue. We identified 3 strains (A1-3) of R. ornithinolytica by colony characteristics, microscopy and Kliger, urease, citrate, indole, catalalase, APUI 20, Vitek2 and motility tests finding that A1 was most productive. By inoculating 0.5ml/100ml R. ornithinolytica A1 on 10g/100 ml potato waste media for 72 hours at 50C and pH 8.5 we obtained a SCP with 24.4% protein, carbohydrate 24,6%, fat 17,9%, ash 21.8% with humidity of 88.6%, DNA 1.235% and RNA 1.012%. The SCP contained very high levels of essential amino acids. Essential and nonessential amino acids constituted 93.9 mg/100g. Copper constituted 0.002, phosphorus 3.90, zinc 0.00056, calcium 0.085, potassium 0.132 and sodium 3.86%.