ISOLATION AND IDENTIFICATION OF PSEUDOMONAS AERUGINOSA CAUSING OTITIS MEDIA AND INVESTIGATION OF SOME GENES OF VIRULENCE FACTORS OF IT IN RAMADI CITY

Abstract

(125) samples of otitis media were collected from patients attending Al-Ramadi General Hospital and private medical clinics. 50 isolates belonging to Pseudomonas aeruginosa bacteria (40%) were isolated and diagnosed from the total samples. Microscopic and phenotypic, biochemical tests were performed, and the diagnosis was confirmed by Phytek2 2, 20 isolates were selected from among the 50 isolates with the most virulence factors for phenotypic virulence, for a PCR test. The results of the qualitative investigation of the genes (FliC, PlcH, GyrB ) of virulence factors showed the presence of these genes in most of the samples in the bacteria P. aeruginosa at a percentage of 90%. The results of the sequencing of nitrogenous bases also confirmed the agreement of the diagnostic results for P. aeruginosa isolates with the isolates registered in NCBI using the MEGA7 program and the percentage of congruence was 100% For each of the gene PlcH isolate No. (8) and the gene GyrB isolate No. (20). As for the FliC gene for isolate No. (5), it showed a number. There were two transversion mutations and two nonequivalent transversion mutations, with a similarity ratio of 99%). In principle, it can be inferred that the plcH, gene and gyrBgenes were found in the same bacterial genus, but in other strains and species with the same sequence of nitrogenous bases.