Cytogenetic Study of Azoospermia and Sever Oligospermia in AlAnbar Governorate

Abstract

Seminal fluid analysis was carried out for 311 samples of subfertile men from Al-Anbar Governorate. Samples were arranged in two groups according to the type of subfertility ; the first group consisted of 217 azoospermia patients )%31.13(, while the second group included 92 oligozoospermia patients )%11.33(, in addition to 40 fertile men as control group. This project also included the study of pedigree analysis for all subfertile men to determine the pattern of inheritance. Hormonal tests (Lotine Hormone ,Follicle Stimulation Hormone ,Prolactine Hormone ,Testosterone Hormone) were carried to eliminate the abnormal patients. Cytogenetic analyses for 61 samples of peripheral blood lymphocytes were performed on 43 subfertile men(azoospermia) and 18 (sever oligospermia), in comparison with 40 cases of fertile men. The results showed the presence of familial continuity with genetic familial relationship in the studied samples. the frequency of families with positive family history were 16 )%3.33( and 3 )%3.11( in azoospermia and oligozoospermia patients, respectively . The study showed the presence of hormonal disorders in 248(80.26%) cases of subfertility, so they were eliminated form the study. The Cytogenetic study of peripheral blood lymphocytes for subfertile men showed the presence of some chromosomal aberrations in(16.28%) and (5.56%)of azoospermia and oligozoospermia patients respectively. However, chromosomal aberrations in the control group were not detected.