انتاج منظم النمو اندول حامض الخليك (IAA) بوساطة البكتريا باستعمال اوساط محلية واختبار كفاءته على نبات فول الصويا

Abstract

This study was included isolation and identification of bacterial isolates for growth promoter production ( Indol Acetic Acid ) by application of local culture and evaluate its efficiency . According to this perpoute bacterial isolates were collected ( nodulan and non nodulen) and examined for their ability to indol production , by used peptone, water , and then serial labrotory experiments were converted to examine ability of selective isolatesfor indol production.The local culture used included dry powder of legume, bean ,soyabean , seed and milk powder ( The culture have high level of treptophans ) . Its also was tested the effect of edition of supplements to the culture such as treptophan ,N. broth , glucose , N and P on indol production optimum condition was included such as pH speed of shaking , Inoculum volume , incubation time ,tempreture , periods of storge to increase production efficincy . The ability of selection isolate for iron chelating natural, phosphate disolveing , Nitrogen fexation were tested. Applied biological expermints has been done in order to evalution the efficincy of indol production in compantion with industrial indol and also the efficincy for the selection isolates and their filteration the effect on the implantation of sow seed and groth of soyabeen which suffering from the prescence and distribution of Rhizopia espelially from lraqi soil . This study have the following results : 1- 18 isolates were obtain cupable for them indol production ( 30 from total isolates) 14 isolats were nodulan bacteria . 2- 8 isolates which have high efficancy in indol production were tested six isolates tended to Rhizopium and two isolate to Pseudomonus in next screening used culture with special condation , two isolates Pssp2S ,Rsp8RA were selected to be used in the following epperinets . 3- When we used 10% of local culture prepared from dry powder of legumes , bean , soyabean seed , milk powder led to increased of indol production significantly ranged from ( 30-50% ). 4- Local culture supplements with peptone , NB , glucose, N and P increased the ability of isolates to indol production(40-65%) significantly . 5- The results of optimum condition study showded the best indol production acharied with R. sp8RA isolat with pH 7.0 ( 39.41 mg IAA / ml and the best shaking speed at 200 / min without erration of media 42.2 mg IAA / ml , the best interaction between the inoculation volume with incubation time with 3 ml / 100 ml of media at 24 hr of incubation ( 42.8 mg IAA / ml ) in microbial population 8.41 Log cfu / ml. 6- This study improved the ability of selection isolates to alchelating compouned CAS from blue to pink colum with 4.5 – 5.8 min. of Ps.sp2S and Rsp8RA respictivly , their isolates have ability to phosphate dissolved in the medium with diametr range dissolving 6.25 – 11.75 mm respectively , and the ,Rsp8RA isolate has ability to nitrogen fixation in the medium with avarge of 3.65 N / L whil the Ps.sp2S doesn't able to nitrogen fixation . 7- Effect of temperature and period of filterated isolates storge on speed of seed germination increased efficancy filterated isolates that stored at 4C and 25C for one day and that stored at 4ْ C for45 days .When it compared with treatment filtrated storge at 25ْ C for 45 days and treatment of industrial indol . The filterated isolates achived in commonly increase in speed and percentage of germination was reached 10 – 20 % in temporal differences 3 – 5 day respectively when compare with control treatment . 8- Result of applied biological experiment showed an increased treatment of two isolates mixture Rsp8RA , PssP2S with their filterated in compare with treatment of isolates mixture only and filtreated only or industrial indol and achaived inclues in : A-Characters of vegetative group that achaived high avarge for branching ( 4.37 ) height of plant ( 81.2 cfu/ ml and leaf area 78.4cm ) ,persente of chlorophyll ( 39.6% ), dry weight (51.2 g) Nitrogen and Phosphours yield in plant ( 491, 1020 mg ) respectively . B- Root characters were achaived high avarge the number of noduls (56.3 noduli) . weight of root group ( 10.4 gm ). c- Soil characters after sowing were achaived high average of nitrogen and phosphorus in soil (166 – 18.4 mg / kg soil) respectively and high microbial population in soil ( 7.82 Log cfu/ ml ).