Influence of Ficus carica and Olea europaea leaves extracts on the mycelial growth of mushrooms in vitro

Abstract

The use of 20% plant leaves extracts included fig (Ficus carica) and olive (Olea europaea) and their mixture 1:1 as an amendment in the solid agar medium (PDA) is beneficial to promote the growth of four mycelial mushrooms. These are Pleurotus ostreatus (Grey oyster mushroom), Pleurotus cornucopiae (Yellow oyster mushroom), Coriolus versicolor (Turkey Tail mushroom), and Ganoderma lucidum (Reishi mushroom). C. versicolor showed better growth reached 67 mm significantly (p b 0.05) on OC medium after five days. While, P. cornucopiae recorded the lowest growth on FC medium reached 35.3 mm. Induction percentage of mycelial growth is changing accord ing to the type of medium and species of fungus. In general, FOH medium exhibited the best percentage of induc tion was 14.89%, followed 12.48% and 9.43% by OH and OC media, while the lower percentages were 5.02% and 5.12% on FH and FC media, respectively. FC medium did not induce growth of P. cornucopiae and C. versicolor. The sterilization by Autoclave and Millipore filter showed different induction percentages. Finally, the extracts of fig and olive were useful to add in the culture media to improve the growth of mycelial mushroom in vitro.