The Kinetic and Thermodynamic Studies on the Binding of 125I-Testosterone to Its Receptors in Uterine Tumors Homogenate

Abstract

Kinetic and thermodynamic studies were carried out on the uterine tumor homogenatebinding to l25l-testost".oua. i'i,',"-"ou.." ofthe association oflt5l-testosterone with its receptor inhuman ute ne tumors at four different temperatures revealed the time and temperature dependency'Association kinetics ildicated pseudo first order kinetics for the binding. Time-courses, scatchard,Van't Hoffs and Arrhenius plots led to the theoretical determination of thermodynamic parametersofbo*r the standard state (i.e., AH', AG', ASo) and transition state (i e , AH+, AG*, AS*)