Study the Relationship between Folic acid and Interleukin 37 with some Anthropometric Measurements and Biochemical Variables in Type 2 Diabetic Patients in Anbar Province

Abstract

Diabetes mellitus (DM) is a complicated persistent sickness related with a state of excessive blood glucose level, or hyperglycemia, going on from deficiencies in insulin secretion, action, or both. In this accurately measure the concentration of Folic acid and IL-37 and explore the possible association that might present between them in T2DM patients and also to explore of the relationship between these parameters with some AMs and some biomarkers. Eighty-eight sample were included in this study, 16 healthy control samples (group A) and 72 patients samples (group B), their ages ranged from 25 to 65 year, collected from General Hit Hospital in Hit city, Ramadi teaching hospital in Al-Anbar Province in the period from December 2019 to March 2020. Serum levels of Folic acid was estimated by Electrochemi luminescence method, serum levels of interleukin-37 was estimated by Enzyme Linked Immune-Sorbent Assay (ELISA), by colorimetric reactions serum levels of urea, creatinine and glucose were estimated, hemoglobin levels was estimated by sandwich immunodetection method. The results which are obtained in this study can be summarized as follows, according to age there was no-significant difference between group A and B, mean of age for group A and B was 45.88 and 50.17 years respectively, mean of BMI was significantly increased in group B (25.67) Kg/m² comparing with group A (22.71) Kg/m², also mean of WHR was significantly increased in group B (1.04) compare with group A (0.77). II Serum glucose levels were increased significantly in group B (199) mg/dL compare with group A (111.3) mg/dL, also significant increase was noticed in HbA1c of group B comparing with group A, mean of HbA1c in Group B and A was 8.926 % and 5.353 % respectively. Non significant difference was noticed in serum levels of urea and creatinine between both groups, mean of blood urea was (38.06) mg/dL in group B compare with (31.03) mg/dL in group A, and mean of creatinine in group B was (0.9389) mg/dL compare with (0.7688) mg/dL in group A. Mean of Folic acid was decreased significantly in group B (7.179) ng/ml compare with group A (12.02) ng/ml, on the other hand, significant increase was noticed in the mean of IL-37 serum levels in group B (1390) pg/ml compare to group A (497.4) pg/ml. According to Pearson Correlation Analysis, strong negative correlation was noticed between Folic acid with IL-37 (r = - 0.392, P = 0.0002), also with creatinine (r = - 0.248, P = 0.0196) and with urea (r = - 0.318, P = 0.0025). Folic acid did not correlate with Age, WHR, BMI, FSG and HbA1c.(r = - 0.128, - 0.185, - 0.175, - 0.212,- 0.179, P = 0.2357, 0.0843, 0.1033, 0.0478, 0.0959 respectively). The correlation between IL-37 with Folic acid was strong negative (r = - 0.392, P = 0.0002). Correlation of IL-37 with WHR, BMI and HbA1c was strong positive correlation (r = 0.503, 0.525, 0.322, P = 0.0001, 0.0001, 0.0023 respectively). and Weak positive correlation with FSG (r = 0.245, P = 0.0214) But did not correlate between IL-37 with age, creatinine and urea (r = 0.141, 0.139, 0.073, P = 0.1888, 0.1957, 0.5017 respectively). III According to Receiver Operating Characteristic Curve Analysis (ROC curve), each of age, creatinine and urea are false positive rates and do not describe the disease case of patients (P ≥ 0.05), on the other hand, each of WHR, BMI, FSG, HbA1c, IL-37 and Folic acid are true positive rates and describe the disease case of patients (P < 0.05).