Study the Effect of Oxidative Stress on Patients with Vitiligo in Anbar Governorate

Abstract

Oxidative stress is an imbalance between oxidants and antioxidants, Which play an important role in initiation and progression of vitiligo. Vitiligo is an acquired, chronic depigmenting disorder of skin. The disease results from the selective loss of melanocytes. The aim of study is to evaluate effect of oxidative stress, heat and light on treatment of patients with vitiligo in AL-Anbar governorate. The study has been included 80 blood samples of vitiligo patients (30 males and 50 females ) and 40 blood samples from healthy individuals (8 males and 32 females ), the date of sampling persists from July 2018 up to January 2019. Many biochemical parameters are performed for all patients and healthy individuals to know the pathogenesis of Vitiligo in addition to questioner list which has been filled for each patient . Their ages are divided into different groups: group (A) ranges between (10-20) years, group (B) ranges between (21-40) years, group (C) ranges between (41-70) years. Family history of vitiligo was positive in a percentage of (30%) of the patients. The duration of disease ranged between 1 month to 20 years . History of seasonal variation of disease was positive in( 60%) patients. Out of these (10%) noticed exacerbation of disease in winter while (50%) in summer season. Bad emotional state exacerbates of about (70%) of vitiligo patients, while the other (30%) does not affect. This study shows that there is no relationship between the occupation, accommodation, most common diet, most common drinks and spiritual side and vitiligo. Hemoglobin Concentration and Packed cells Volume determination of patient's group were significantly lower than those of control group with ( P ≤ 0.05 ). The results obtained from this study showed a significant increase in Malondialdehyde (MDA) concentration with ( P ≤ 0.05 ) in vitiligo patients at comparison with control group and showed that there are no significant differences with ( P ≥ 0.05 ) in the concentrations of total bilirubin in vitiligo patients at comparison with control group and showed significant increase in the concentrations of uric acid with (P ≤ 0.05) in vitiligo patients at comparison with control and showed significantly increase in glutathion concentration with ( P ≤ 0.05 ) in vitiligo patients at comparison with control group. The statistical analysis for the determination some of vitamins showed that there are no significant differences in the concentrations of vitamin E and C with ( P ≥ 0.05 ) in vitiligo patients at comparison with control group. The statistical analysis for the determination some of trace elements showed a significant increase in the concentrations of Zn and Cu with (P ≤ 0.05) in vitiligo patients at comparison with control group. This study shows that there is no correlation relationship between oxidants MDA and antioxidants ( vitamin C, vitamin E, Zn ). But it shows a negative correlation between MDA and Glutathione (r=-0.447) at significant (p≤0.01) and a positive correlation between MDA and Cu (r=0.409 ) at significant (p≤0.01). Lipids profile is determined and the statistical analysis results show that serum cholesterol, triglycerides, high density lipoprotein cholesterol (HDL-cholesterol), low density lipoprotein cholesterol (LDL-cholesterol) and very low density lipoprotein cholesterol (VLDL-cholesterol) were no significant differences than those in control group with ( P ≥ 0.05 ). The results of thyroid hormones determination were in the patient's group T3 and T4 were no significant differences than those in control group ( P ≥ 0.05 ).while TSH was significantly higher than those in control group ( P ≤ 0.05 ). This study evaluated the effect of Narrow-band ultraviolet B (NB UVB) on the 20 patients ( 12 women and 8 men ) with vitiligo. The response of 80-90%was given grade excellent and response of 70-80% was given grade good and response of 60-70% was given grade moderate, while response of 50-60% was given grade mild and response of 40-50% was given grade poor.