Spectroscopic studies in UV region of different purified isoforms of testosterone receptors in human ovarian tumor homogenates

Abstract

The aim of this study is to characterize the testosterone receptors spectrophotometrically in ovarian tissue homogenates of patients with benign and malignant ovarian tumors. Two groups of ovarian tumor patients were included in this study .Group I contained 31 patients with benign ovarian tumor .Group II consisted of 18 patients with ovarian cancer.Spectroscopic characterization in the UV region was carried out on purified testosterone receptors in human ovarian tumors and the effects of pH and polarity on the spectra were also studied.Spectrophotometric pH titration and several spectral changes were obtained in the presence of different polar and non-polar solvents, like the alteration of max position and intensities of protein spectrum, and the appearance of new chromophores on the surface of protein molecule .These chromophores where embedded in an interior region of the protein in the absence of the solvent. The difference in pH and polarity of the solvents is very important thing to characterize the protein molecules spectrophotometrially because they change the positions and values of molecules λmax in the UV region.