دراسة تصنيفية وجزيئية لبعض عوامل الضراوة للبكتريا الممرضة

Abstract

Out of 302 clinical samples 50 isolates were identified as Proteus mirabilis, isolated from various sources, their incidence occuring in the following percentage: 42 % were isolated from the ear, 38% from urine, 10% from stool, 6% from wounds and 2% from other sources. Specimens were collected from hospitalized patients of both sexes in Baghdad. The P. mirabilis isolates were subjected to forty one biochemical tests which were further analyzed by using statistical analysis by computer programming for calculating the similarity rate using the Ssm (Simple matching coefficient). Cluster analysis (dendrogram) indicated the distribution of the 50 isolates in to three biotypes, I, II, and III. Further tests were carried out to detect virulence factor production and the results were as follows; all isolates were urease producers and had the ability to swarm, 92% were protease producers on skim milk medium but only 84%, showed protease activity on casein medium. All isolates were gelatinase producers but none lysed elastin and collagen.