"Characterizations of physical properties of synthesized silver nanoparticles and their effect on the levels of parameters in the blood of female rats "

Abstract

"Silver nanoparticles were synthesized by using a simple method namely cold atmospheric plasma which is generated by argon gas. The silver nanoparticles were prepared by using an aqueous solution of (AgNO3) with different concentrations (0.5, 1, 1.5, 2) mM. The effects of concentration and exposure time on particle size were investigated. The UV-VIS spectrum for Ag nanoparticles solution shows that it has peaks in the visible region located between (390-422) nm , this indicates the formation of Ag nanoparticles due to the surface Plasmon resonance. The peaks of the absorption spectrum depend on the concentration and exposure time. The full width at half maxima is increased by increasing exposure time and the concentration of silver nitrate (Ag NO3). The maximum absorbance was found at a time (6 min) of plasma exposure to AgNO3 concentration (1 mM). The XRD pattern of prepared silver nanoparticles as afunction of exposure time (2, 4, 6, 8, 10 min) at a concentration (1 mM) of AgNO3 shows a strong peak is observed at (2Ѳ= 38.1°) with a crystalline size 23.87 nm , the other peak (2Ѳ = 44.27°) with a crystalline size 37.94 nm , assigned to the formation of cubic silver nanoparticle, the peak became more clear and defined at time (6 min) and that the Ag was crystalline and face-centered cubic, while the XRD pattern of silver nanoparticles at different concentrations (0.5, 1.5, 2 mM) of AgNO3 with time (4 min) shows strong peaks are detected at (2Ѳ = 38.1°), (2Ѳ = 44.27°), and (2Ѳ = 64.42°). These obtained peaks agree well with the cubic structure. The FESEM measurement of Ag nanoparticles showed a homogeneous pattern structure , the obtained images of different times (2, 4, 6, 8, 10 min) at a concentration (1 mM) of AgNO3 reveal that the particle size is variable and within the range of (17−55) nm and that the Ag was spherical nanoparticles. The FESEM microscopic images of Ag nanoparticles of different concentrations at plasma exposure time (4 min) show that the particle size is variable and is within the range of (30−74) nm , with a spherical shape. EDX was used to find the elemental composition of the samples. The EDX of Ag NPs revealed that the presence of pure silver was the major constituent element compared to the sillicon attributed to the substrate and there were no impurities , the synthesized Ag NPS show strong absorption in the range of 0.5–3 KeV. In the application , the effect of the Ag nanoparticles has been studied on the levels of Fe, Na, Ca, Cl, Creatine, Urea, ALB, GPT, CK and LDH in the blood of female rats. Rats were divided into (4) groups,each group containing (5)animals.The first group was a control group. The second group was dosed with (170 ppm) Ag nanoparticles ; the third group was dosed with (340 ppm) Ag nanoparticles, and the fourth group was dosed with (680 ppm) Ag nanoparticles . The time between one dose and another was one week. Every week, the blood levels were analyzed. The Fe , Ca concentrations in the blood of rats increased when the exposure period and the concentrations of silver nanoparticles were increased compared to the control group. There were no significant differences in the effects of silver nanoparticles on (Na , Cl , creatine ) concentrations in the blood serum with increasing doses and time of treatment. There were significant differences in the levels of albumin protein , urea, GPT ,CK and LDH in the blood serum of rats treated with different concentrations of silver nanoparticles according to the duration of exposure. "