Detection of H.pylori cagA gene in patient's with gastroduodenal disease

Abstract

Helicobacter pylori infection is common in the developing countries. The cagA gene is a marker of pathogenicity island (PAI) in H. pylori. The aim of this study was to determine the prevalence of cagA among gastroduodenal disease (peptic ulcer and non-ulcer dyspepsia) in patients of Ramadi-city. A total of 81 gastric biopsy samples, gastric ulcer 10 (12.30% ) , duodenal ulcer 18 (22.20% ) and non-ulcer dyspepsia 53 (65.40% ) by endoscopic examination . cagA gene of H. pylori was assessed using polymerase chain reaction (PCR). The cagA gene was detected by PCR technique in 3 from 10 biopsy specimens culture positive, urease test was showed 7(8.64%) patients, 2(2.5%) patients and 48(59.26%)patients after (2 hr, 6hr and 20 hr) respectively, ELISA positive result was showed 12(14.8%) patients. Urease test is easy test , inexpensive and can be use in endoscopy unit, but non-specific, culture is gold stander for detection of H.pylori, ELISA technique use for detection of anti-H.pylori IgG , ELISA technique widly used for epidemiology studies. PCR assay used for detection of cagA gene. Using PCR to detect cagA gene from culturecolony.Thepotentialadvantagesof PCRincludehighspecificity,quickresultsandtheabilitytotype bacteria without the requirement for special transport conditions..