Histochemical study of effect of black seed oil extract (Nigella Stavia) on liver tissue of fat feeding male rabbits

Abstract

This study aimed to investigate the histochemical effect of black seed oil extract on liver tissue in fat feeding rabbits. The study included fifty rabbit males were divided equally into 5 groups: 1. Control group which treated with normal saline as control (C), and fed normal diet. 2. The second group (ch) was fed normal diet with 1% cholesterol for 90 days. 3. The third group (chn) was treated with oil extract of Black seed as 500 mg/ kg of body weight for each animal by oral tube, in addition to 1% cholesterol for 90 days. 4. The fourth group (Nch) was treated with oil extracts of Black seeds as 500 mg/kg by gastric tube for 30 days, and then fed cholesterol only for 60 days. 5. The fifth group (N) was treated with oil extracts of Black seeds as 500 mg/kg by gastric tube mixed with normal diet for 90 days. The animals dissected in regular times to get the liver samples for histological and histochemical study by use special stains. Histopathological study was revealed fibrosis in liver tissue and the proliferation of bile ducts in (ch) group while the group Nch and N did not show any tissue changes significantly. The chn group was show simple changes which returned to normal at the end of experiment. These results were detected to role of black seed oil extract in reduced the harmful effect of cholesterol. This study shows the role of black seed oil in reduced influence of cholesterol on the tissues.